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Recombinant Human OSM, Tag Free

Catalog No.
PH1007
 
Grouped product items
SizePriceStock Qty
10ug
$90.00
Ship with 5-10 days
50ug
$264.00
Ship with 5-10 days

Tel: +1-832-696-8203

Email: [email protected]

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Background

Oncostatin M is also known as OSM, is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Of these cytokinesit most closely resembles leukemia inhibitory factor (LIF) in both structure and function. However, it is as yet poorly defined and is proving important in liverdevelopment, haematopoeisis, inflammation and possibly CNS development. It is also associated with bone formation and destruction. OSM signals through cellsurface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.Oncostatin M (OSM) was previoustly identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported thatOSM, like LIF, IL-6 and G-CSF, has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degree but is not stable at 90 degrees. The threedimensional structure of human OSM has been solved to atomic resolution, confirming the predicted long chain four helix bundle topology. Comparing this structure with the known structures of other known LC cytokines shows it to be most closely related to LIF.

Reference:
1. Tanaka M, Miyajima A. Oncostatin M, a multifunctional cytokine. Rev Physiol Biochem Pharmacol. 2003;149:39-52. doi: 10.1007/s10254-003-0013-1. Epub 2003 Jun 17. PMID: 12811586.
2. Walker EC, McGregor NE, Poulton IJ, Solano M, Pompolo S, Fernandes TJ, Constable MJ, Nicholson GC, Zhang JG, Nicola NA, Gillespie MT, Martin TJ, Sims NA. Oncostatin M promotes bone formation independently of resorption when signaling through leukemia inhibitory factor receptor in mice. J Clin Invest. 2010 Feb;120(2):582-92. doi: 10.1172/JCI40568. Epub 2010 Jan 4. PMID: 20051625; PMCID: PMC2810087.
3. Auguste P, Guillet C, Fourcin M, Olivier C, Veziers J, Pouplard-Barthelaix A, Gascan H. Signaling of type II oncostatin M receptor. J Biol Chem. 1997 Jun 20;272(25):15760-4. doi: 10.1074/jbc.272.25.15760. PMID: 9188471.
4. Deller MC, Hudson KR, Ikemizu S, Bravo J, Jones EY, Heath JK. Crystal structure and functional dissection of the cytostatic cytokine oncostatin M. Structure. 2000 Aug 15;8(8):863-74. doi: 10.1016/s0969-2126(00)00176-3. PMID: 10997905.

Description

Synonym MGC20461, Oncostatin M.
Gene ID 5008
Accession # NP_065391.1
Molecular Characterization Ala 26 - Arg 252
M.Wt 25.8 kDa
Source 293T cells
Bio Activity Determined by the dose-dependent stimulation of murine CTLL-2 cells:
ED50:< 0.1 ng/mL
Specific activity: > 1x107units/mg.

Quality Control

Quality Control & MSDS

View current batch:
    Purity ≥ 95%, by SDS-PAGE and HPLC.
    Endotoxin Level: < 0.1 ng/μg
  • Datasheet
 

Components and Storage

Formulation The protein is dissolved in PBS buffer.
Storage

This product is stable after storage at:

4°C for 1 week;

-20°C for 3 months.

Please avoid repeated freeze-thaw cycles.