Acetoacetate Colorimetric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Acetoacetate (AcAc) is the simplest β-keto acid and is one of the three ketone bodies. Acetoacetate is produced through condensation of two molecules of acetyl-CoA in liver mitochondria. Acetoacetate can be decarboxylated to produce acetone (CH3)2CO or enzymatically reduced to 3-β-hydroxybutyrate (β-HB). Ketone bodies (β-HB: 78%; AcAc: 20%; (CH3)2CO: 2%) are primarily used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies is found in patients with severe starvation, alcoholism or Type I diabetes. Using the traditional method, AcAc levels can be qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. The Acetoacetate Colorimetric Assay Kit provides a specific and sensitive way for detection of AcAc levels in various body fluids based on colorimetric method. The kit modified the principle of the traditional method. In the assay, AcAc reacts with a substrate to yield a colored product that can be measured at 550 nm. The assay is specific for AcAc and does not detect β-HB. The kit can detect as low as 25 µM AcAc in human blood, urine and other body fluids.
Features & Properties
|Features||Specific for acetoacetate and does not detect 3-β-hydroxybutyrate; The assay can detect less than 25 µM of acetoacetate in various sample types.|
|Storage Conditions||Store at -20°C.|
|Kit components||Acetoacetate Assay Buffer, Acetoacetate Standard (Lyophilized), Acetoacetate Substrate|