Adiponectin (human) Elisa Assay Kit

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Adipose tissue secretes various biologically active soluble factors that regulates metabolism of glucose and fatty acid. The Adiponectin (human) Elisa Assay Kit is an enzyme-linked immunosorbent assay for quantitative analysis of andiponectin in human serum, plasma and tissue/cell culture supernatants etc. In this assay, human adiponectin specific monoclonal antibody has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells. The adiponectin present is than attached to the immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors generated is proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Features | Simple procedure; Fast and convenient. |
Shipping | Gel pack. |
Storage Conditions | Store at +4°C. |
Kit components | Antibody coated 96-well plate (12 x 8-well strips), 10X Wash concentrate, 50 mL, 5X Diluent, 50 mL, Secondary antibody, 12 mL, 100X Detector, 150 µL, Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized, QC sample = positive control having 7-11 µg/mL human adiponectin/ gACRP30, 1 vial, lyophilized, Substrate I, 6 mL, Substrate II, 6 mL, Stop solution, 12 mL |