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Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit

Catalog No.
K2218
Detects alpha-ketoglutarate dehydrogenase activity.
Grouped product items
SizePriceStock Qty
100 assays
$536.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Pyruvate Dehydrogenase (PDH) plays an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH forms a well-characterized enzyme complex. In the presence of NAD+ and CoA, PDH converts pyruvate into acetyl-CoA. PDH also links glycolysis metabolic pathway to the citric acid cycle. PDH activity is inhibited by high ratios of intracellular Acetyl-CoA/CoA, NADH/NAD+ or ATP/ADP. In humans, PDH deficiency inhibits mitochondrial function and is involved in neurodegenerative diseases. PDH deficiency is X-linked and results in 2 forms of abnormality: seizure and/or neuropathological spasm (a neurological form) and lactic acidosis (a metabolic form). PDH is also a target of oncogene-induced senescence. Activation of PDH can increase respiration and redox stress, and enhance pyruvate utilization.

 The Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection PDH activity in various samples based on colorimetric method. In the assay, PDH changes pyruvate to an intermediate which reduces the developer to a colored (450 nm) product. The kit can detect PDH activity lower than 0.1 mU in a variety of samples.

Features & Properties

FeaturesSimple, rapid & convenient; Can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Storage

Store at -20°C.

Overview

Kit componentsPDH Assay Buffer, PDH Substrate (Lyophilized), PDH Developer (Lyophilized), NADH Standard (Lyophilized), PDH Positive Control