Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
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Phos Binding Reagent Acrylamide
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A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pyruvate Dehydrogenase (PDH) plays an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH forms a well-characterized enzyme complex. In the presence of NAD+ and CoA, PDH converts pyruvate into acetyl-CoA. PDH also links glycolysis metabolic pathway to the citric acid cycle. PDH activity is inhibited by high ratios of intracellular Acetyl-CoA/CoA, NADH/NAD+ or ATP/ADP. In humans, PDH deficiency inhibits mitochondrial function and is involved in neurodegenerative diseases. PDH deficiency is X-linked and results in 2 forms of abnormality: seizure and/or neuropathological spasm (a neurological form) and lactic acidosis (a metabolic form). PDH is also a target of oncogene-induced senescence. Activation of PDH can increase respiration and redox stress, and enhance pyruvate utilization.
The Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection PDH activity in various samples based on colorimetric method. In the assay, PDH changes pyruvate to an intermediate which reduces the developer to a colored (450 nm) product. The kit can detect PDH activity lower than 0.1 mU in a variety of samples.
Features & Properties
|Features||Simple, rapid & convenient; Can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||PDH Assay Buffer, PDH Substrate (Lyophilized), PDH Developer (Lyophilized), NADH Standard (Lyophilized), PDH Positive Control|