StayBrite Highly Stable ATP Bioluminescence Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Luciferase is a useful tool for sensitive measurement of ATP levels, but the conventional method are usually unstable. The StayBrite Highly Stable ATP Bioluminescence Assay Kit utilize a highly stable Luciferase (rLucHS) which has enhanced stability and sensitive with relatively more effective pH range. rLuckHS is prominently more active than Photinus luciferase and is stable for weeks at room temperature and over 60 mins at 37°C. The detection limit can be as low as 10 fmol/assay (1 nM). rLucHS specific activity is approx. 5 x 10ᶦᶦ RLU/mg protein. This assay can also be fully automated for high throughput (1 second/sample) and is highly sensitive ATP production or consumption in various enzymatic reactions.
Features & Properties
|Features||Simple procedure; Takes approx. 20-30 minutes; Fast and convenient; Stable for >10 hours at room temperature and >60 minutes at 37°C; The stable reagent provides more accuracy than any other ATP assay kits.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||StayBrite Reaction Buffer, StayBrite Reconstitution Buffer, StayBrite Enzyme Mix (lyophilized) , ATP Standard (MW 551) (lyophilized)|