Caspase-9 Inhibitor Drug Screening Kit (Fluorometric)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays an important role in cell apoptosis. Inhibition of caspases can delay apoptosis, indicating a potential role in drug screening efforts. Caspase-9 is a member of the caspase family. Caspase-9, as well as caspase-2, -8 and -10, is an initiator caspase. Caspase-9 is involved in the mitochondrial death pathway and is activated during apoptosis. The activation of JNK/SAPK stress signaling pathways induces the release of cytochrome c from mitochondria and activation of apaf-1, which then cleaves caspase-9 into the active form. The active Caspase-9 cleaves and activates Caspase-3 and Caspase-7, which then cleave poly ADP ribose polymerase.
The Caspase-9 Inhibitor Drug Screening Kit (Fluorometric) provides a simple, fast and convenient way for screening of caspase-9 inhibitors based on fluorometric method. The synthetic peptide substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm). While cleavage of LEHD-AFC by active caspase-9, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be quantified by a fluorecence microtiter plate reader or a fluorometer. Inhibitors can be directly added to the reaction and the efficacy of inhibition of caspase-9 activity is determined by comparison of the fluorescence intensity in samples without and with the testing inhibitors. The kit is suited to perform directly in microtiter plates.
Features & Properties
|Features||Fast and convenient; Simple one-step procedure; Takes only 1-1.5 hours; Inhibition of caspase-9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
- View current batch:
|Kit components||2X Reaction Buffer, Caspase Substrate LEHD-AFC (1 mM), DTT (1 M), Active Caspase-9 (Lyophilized), Caspase Inhibitor, Z-VAD-FMK (2 mM)|