DPP4 Activity Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Phos Binding Reagent Acrylamide
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DPP4 (Dipeptidyl peptidase 4, also known as T-celll activation antigen CD26) is a membrane expressed peptidase which exhibits multiple effects throughout the body. It plays an important role in glucose metabolism. It mediates the degradation of incretins (e.g. GLP-1) and as a target for type II diabetes and cancer treatment. In the DPP4 Activity Fluorometric Assay Kit, DPP4 cleaves a substrate to generate a quenched fluorescent group, AMC (7-Amino-4-Methyl Coumarin, Ex/Em = 360/460 nm). This kit provides a fast, selective and robust way for high throughput activity screening of DPP4. The detection limit is 3 µU per well.
Features & Properties
|Features||Simple procedure; Takes approx. 40 minutes; Fast and convenient; Sensitive, reliable HTS assay.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||DPP4 Assay Buffer, DPP4 Substrate (H-Gly-Pro-AMC), DPP4 Positive Control, AMC Standard (1 mM), DPP4 Inhibitor (Sitagliptin)|