Thioredoxin Reductase Activity Colorimetric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Phos Binding Reagent Acrylamide
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Thioredoxin reductase (TrxR) is an ubiquitous enzyme that mediates various cellular processes (e.g. p53 activity, cell growth and defense of oxidative damage). The mammalian TrxR downregulate non-disulfide substrates such as lipoic acids, selenite and hydrogen peroxide etc. Thioredoxin Reductase Assay Kit offers a fast and easy colorimetric assay for measuring TrxR activity in different sample. In this assay, TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), produce an intense yellow color ((λmax = 412 nm.). Because glutathione peroxidase and glutathione reductase can also reduce DtNB in crude biological ample, TrxR specific inhibitor is used to detect the TrxR specific activity. Two assays are carried out: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample with the TrxR specific inhibitor. The DTNB reduction by TrxR is the difference between the two results.
Features & Properties
|Features||Simple procedure; takes ~ 30-40 minutes Fast and convenient|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||TrxR Assay Buffer TNB Standard DTNB NADPH TrxR Positive Control TrxR Inhibitor|