Cathepsin D Inhibitor Screening Kit (Fluorometric)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apoptosis is often mediated by the traditional caspase-mediated cleavage cascade. In addition, alternative proteolytic enzymes such as the lysosomal cathepsin proteases can also initiate or propagate proapoptotic signals. Cathepsins are lysosomal proteases that play an important role in mammalian cellular turnover such as bone resorption. Cathepsins are often used as sensitive markers in a variety of toxicological investigations. Cathepsin D is a lysosomal aspartyl protease belonging to the peptidase A1 family and is used as a marker of breast cancer tumor.
The Cathepsin D Inhibitor Screening Kit (Fluorometric) provides a simple, fast and convenient way for screening of cathepsin D inhibitors based on fluorometric method. The assay utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2 labeled with MCA. While cleavage of the synthetic substrate by cathepsin-D, the released fluorescence (Ex/Em = 328/460 nm) can be easily quantified using a fluorecence microtiter plate reader or a fluorometer. The relative efficacy of the test inhibitor is compared to the positive control inhibitor Pepstatin A (IC50 < 0.1 nM). The kit is suitable for high throughput screening (HTS) and can be assayed in 96-well plate.
Features & Properties
|Features||Fast, robust and convenient. Simple one-step procedure; takes only 1 hour. Cathepsin-D inhibitor screening assay is a fast and robust assay to screen potential inhibitors. Positive control inhibitor, Pepstatin A is included in the kit.|
|Storage Conditions||Store at -20℃.|
Quality Control & DataSheet
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|Kit components||CD Reaction Buffer CD Substrate (1 mM) Cathepsin D, human (lyophilized) Pepstatin A (1 mM in DMSO)|