Cathepsin L Activity Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apoptosis is often mediated by the traditional caspase-mediated cleavage cascade. In addition, alternative proteolytic enzymes such as the lysosomal cathepsin proteases can also initiate or propagate proapoptotic signals. Cathepsins are lysosomal proteases that play an important role in mammalian cellular turnover such as bone resorption. Cathepsins are often used as sensitive markers in a variety of toxicological investigations. Cathepsin L is a lysosomal cysteine protease belonging to the peptidase C1 family and is involved in the initiation of protein degradation.
The Cathepsin L Activity Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of cathepsin L activity based on fluorometric method. The assay utilizes the preferred cathepsin-L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). While cleavage of the synthetic substrate FR-AFC by cathepsin-L in cell lysates or other samples, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be easily quantified using a fluorecence microtiter plate reader or a fluorometer.
Features & Properties
|Features||Fast and convenient. Simple one-step procedure; takes only 1-2 hours. Cathepsin-L assay is straightforward, and can be adapted to 96-well plate assays.|
|Storage Conditions||Store at -20℃.|
Quality Control & DataSheet
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|Kit components||CL Buffer DTT Cathepsin L Positive Control CL Substrate Ac-FR-AFC (10 mM) CL Inhibitor (1 mM)|