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Cathepsin B Activity Fluorometric Assay Kit

Catalog No.
K2151
Detects cathepsin B activity, sensitive.
Grouped product items
SizePriceStock Qty
100 assays
$422.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Apoptosis is often mediated by the traditional caspase-mediated cleavage cascade. In addition, alternative proteolytic enzymes such as the lysosomal cathepsin proteases can also initiate or propagate proapoptotic signals. Cathepsins are lysosomal proteases that play an important role in mammalian cellular turnover such as bone resorption. Cathepsins are often used as sensitive markers in a variety of toxicological investigations. Cathepsin-B is an lysosomal cysteine protease belonging to the peptidase C1 family.

The Cathepsin B Activity Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of cathepsin B activity based on fluorometric method. The assay utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). While cleavage of the synthetic substrate RR-AFC by cathepsin-B in cell lysates or other samples, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be easily quantified using a fluorecence microtiter plate reader or a fluorometer.

Product Citation

Chemical Properties

StorageStore at -20°C.
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Features & Properties

ShippingGel pack.

Quality Control

Quality Control & DataSheet

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Related Biological Data

Cathepsin B Activity Fluorometric Assay Kit

Related Biological Data

Cathepsin B Activity Fluorometric Assay Kit

Related Biological Data

Cathepsin B Activity Fluorometric Assay Kit

Related Biological Data

Cathepsin B Activity Fluorometric Assay Kit

Related Biological Data

Cathepsin B Activity Fluorometric Assay Kit