Cathepsin B Activity Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apoptosis is often mediated by the traditional caspase-mediated cleavage cascade. In addition, alternative proteolytic enzymes such as the lysosomal cathepsin proteases can also initiate or propagate proapoptotic signals. Cathepsins are lysosomal proteases that play an important role in mammalian cellular turnover such as bone resorption. Cathepsins are often used as sensitive markers in a variety of toxicological investigations. Cathepsin-B is an lysosomal cysteine protease belonging to the peptidase C1 family.
The Cathepsin B Activity Fluorometric Assay Kit provides a sensitive, simple and convenient way for detection of cathepsin B activity based on fluorometric method. The assay utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). While cleavage of the synthetic substrate RR-AFC by cathepsin-B in cell lysates or other samples, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be easily quantified using a fluorecence microtiter plate reader or a fluorometer.
- 1. Gong Z, Zhao S, et al. "Curcumin alleviates DSS-induced colitis via inhibiting NLRP3 inflammsome activation and IL-1β production." Mol Immunol. 2018 Dec;104:11-19. PMID:30396035
- 2. Shengnan Zhao, Zizhen Gong, et al. "Deoxycholic acid-Mediated Sphingosine-1-Phosphate Receptor 2 Signaling Exacerbates DSS-induced Colitis through Promoting Cathepsin B Release."
|Storage||Store at -20°C.|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
Features & Properties
Quality Control & DataSheet
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