Caspase-12 Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-12 is an inflammatory caspase that is involved in processing and activating inflammatory cytokines such as interleukin 1 and interleukin 18. Inhibition of caspase-12 leads to a stronger inflammatory reaction to bacterial pathogens.
Caspase-12 Fluorometric Assay Kit provides a highly sensitive, simple and convenient way for detecting the ATAD-dependent caspase activity based on detection of cleavage of substrate ATAD-AFC. ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of ATAD-AFC by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence from an apoptotic sample with an uninduced control determines the fold increase in caspase-12 activity.
Features & Properties
|Features||Fast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
- View current batch:
|Kit components||Cell Lysis Buffer, 2X Reaction Buffer, ATAD-AFC Substrate, DTT (1 M)|