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Caspase-12 Fluorometric Assay Kit

Catalog No.
K2150
Detects ATAD-dependent caspase-12 activity, highly sensitive.
Grouped product items
SizePriceStock Qty
25 assays
$183.00
In stock
100 assays
$435.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-12 is an inflammatory caspase that is involved in processing and activating inflammatory cytokines such as interleukin 1 and interleukin 18. Inhibition of caspase-12 leads to a stronger inflammatory reaction to bacterial pathogens.

Caspase-12 Fluorometric Assay Kit provides a highly sensitive, simple and convenient way for detecting the ATAD-dependent caspase activity based on detection of cleavage of substrate ATAD-AFC. ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of ATAD-AFC by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm) that can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence from an apoptotic sample with an uninduced control determines the fold increase in caspase-12 activity.

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Storage

Store at -20°C.

Overview

Kit componentsCell Lysis Buffer, 2X Reaction Buffer, ATAD-AFC Substrate, DTT (1 M)