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Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

Catalog No.
Sensitive Assay, HTS
Grouped product items
SizePriceStock Qty
100 assays
In stock

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Myeloperoxidase (MPO) is a peroxidase enzyme that expressed most abundantly in neutrophil granulocytes. It is a liposomal protein located in the azurophilic granules of the neutrophil. MPO catalyzes the conversion of hydrogen peroxide and alide ions to cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms.. MPO oxidizes tyrosine to a tyrosyl radical utilizing hydrogen peroxide as an oxidizing agent. In Myeloperoxidase (MPO) Fluorometric Activity Assay Kit, MPO catalyzes reaction of H₂O₂ and NaCl to produce NaClO that subsequently reacts with the Aminophenyl fluorescein (APF) to generate detectable strong fluorescence (Ex/Em = 485/525 nm). This kit offers a reliable and sensitive way for high throughput activity assay of MPO. The detection limit can be as low as 0.5 μU per well.

Features & Properties

FeaturesSimple procedure Fast and convenient Sensitive assays for measuring MPO as low as 0.05 mU per well
ShippingGel pack
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Kit componentsAssay Buffer MPO Substrate Stock MPO Probe Fluorescein Standard (1 mM) MPO Positive Control