Myeloperoxidase (MPO) Fluorometric Activity Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Myeloperoxidase (MPO) is a peroxidase enzyme that expressed most abundantly in neutrophil granulocytes. It is a liposomal protein located in the azurophilic granules of the neutrophil. MPO catalyzes the conversion of hydrogen peroxide and alide ions to cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms.. MPO oxidizes tyrosine to a tyrosyl radical utilizing hydrogen peroxide as an oxidizing agent. In Myeloperoxidase (MPO) Fluorometric Activity Assay Kit, MPO catalyzes reaction of H₂O₂ and NaCl to produce NaClO that subsequently reacts with the Aminophenyl fluorescein (APF) to generate detectable strong fluorescence (Ex/Em = 485/525 nm). This kit offers a reliable and sensitive way for high throughput activity assay of MPO. The detection limit can be as low as 0.5 μU per well.
Features & Properties
|Features||Simple procedure; Fast and convenient; Sensitive assays for measuring MPO as low as 0.05 mU per well.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||Assay Buffer, MPO Substrate Stock, MPO Probe, Fluorescein Standard (1 mM), MPO Positive Control|