Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
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Phos Binding Reagent Acrylamide
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Choline is a water-soluble nutrient. Acetylcholine (ACh) acts as a neurotransmitter in the autonomic nervous system (ANS). Choline and ACh play important roles in many biological processes.
The Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit provides a highly sensitive, fast and convenient way for detection of choline and acetylcholine in various biological samples such as blood, cells, fermentation media and culture media based on colorimetric and fluorometric method. In the assay, free choline is oxidized to betaine via the intermediate betaine aldehyde. The reaction generates products which react with Choline Probe to yield fluorescence (Ex/Em 535/587 nm) and color (λ = 570 nm). Acetylcholine is converted to choline by acetylcholinesterase. The assay can detect choline and acetylcholine (total choline-free choline). The kit can detect 10 pmol-5 nmol of choline or acetylcholine without pretreatment or purification of samples.
Features & Properties
|Features||Fast and convenient; Simple procedure; Takes approx. 40 minutes; Kit provides all necessary buffers and reagents for an accurate assay of Choline/Acetylcholine in various biological samples.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||Choline Assay Buffer, Choline Probe, Choline Enzyme Mix, Acetylcholinesterase, Choline Standard (5 μmol)|