Mitochondrial DNA Isolation Kit

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The high mutation rate of Mitochondrial DNA (mtDNA) has been linked to some diseases such as Alzheimer’s diseases, diabetes and muscle disorders. Isolation and quantification of mtDNA is an important method for studying the links between mtDNA and diseases. This kit offers a simple and effective tool for isolating mtDNA from various cells and tissues with high without genomic DNA contaminations. The purified mtDNA can be applied into enzyme manipulations, cloning and PCR etc.
Features | Simple procedure; Takes less than 2 hours; Fast and convenient; The kit provides unique formulations of buffers and reagents for isolating a highly enriched mitochondrial fraction. Mitochondrial DNA are then isolated using reagents included in the kit. |
Shipping | Gel pack. |
Storage Conditions | Store at -20°C. |
Kit components | 5X Cytosol Extraction Buffer, Mitochondrial Lysis Buffer, Enzyme B Mix (lyophilized), TE Buffer |