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Cytochrome c Apoptosis Assay Kit

Catalog No.
K2104
 
Grouped product items
SizePriceStock Qty
100 assays
$586.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Cytochrome c is located between the inner and the outer mitochondrial membranes. Once activated by the apoptosis stimulus, cytochrome c is released from mitochondria into cytosol where it couples to Apaf-1. The cytochrome c/Apaf-1 complex further activates the downstream caspases. The Cytochrome c Releasing Apoptosis Assay Kit gives an efficient way for sensing cytochrome c releasing from mitochondria into cytosol under apoptosis. The kit utilizes a special formulation of reagents to isolate enriched mitochondrial fraction from cytosol. The protocol is simple to perform without unltracentrifugations and toxic chemicals. The cytochrome c antibody provided in the kit is then used to determine the Cytochrome c releasing from mitochondria into cytosol by Western blotting.

Features & Properties

FeaturesSimple procedure; Takes approx. 3 hours; Fast and convenient; Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.
ShippingGel pack.
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

View current batch:
 

Storage

Store at -20°C.

Overview

Kit componentsMitochondria Extraction Buffer, 5X Cytosol Extraction Buffer, DTT (1 M, 500X Protease Inhibitor Cocktail, Anti-Cytochrome c mouse mAb (0.2 mg/mL)