ADP/ATP Ratio Bioluminescence Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The changes in ADP/ATP ratio is used to recognized the different states of the cell death and viability. Levels of ATP and ADP levels are increased in proliferating cells but decreased in apoptotic cells. The ADP/ATP Ratio Assay Kit detects ATP and ADP levels by bioluminescent for a fast screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. This kit utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can measured using a luminometer or Beta Counter. ADP level is measured by its conversion to ATP that is subsequently detected using the same reaction. The assay can be fully automatic for high throughput and is highly sensitive (detects 100 mammalian cells/well).
Features & Properties
|Features||Simple one-step procedure; Takes only 30 minutes; Fast and convenient.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||Nucleotide Releasing Buffer, ATP Monitoring Enzyme, ADP Converting Enzyme, Enzyme Reconstitution Buffer|