Nitric Oxide Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nitric oxide is essential in neurotransmission, vascular regulation, immune response and apoptosis. Because Nitric oxide is rapidly converted into nitrite and nitrate, the NO production can be quantitatively determined by the total concentration of nitrite and nitrate. This kit is a simple and accurate two-step measurement of total nitrate/nitrite concentration. Nitrate reductase converts nitrate into nitrite first, and then nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The total nitric oxide production is proportional to the fluorescent intensity. The kit has been tested with culture media, plasma, and tissue homogenates.
Features & Properties
|Features||Simple procedure; takes only 2-3 hours Fast and convenient|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||Assay Buffer Enzyme Cofactor Enhancer Nitrate Reductase Nitrate Standard Nitrite Standard DAN Probe Sodium Hydroxide Microtiter plate Plate Cover|