Mitochondrial Apoptosis Detection Fluorometric Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The first intracellular action that happens after the activation of apoptosis is the disruption of the mitochondrial transmembrane potential. By detecting the fluctuation in the mitochondrial transmebrane potential, this kit is an easy and fluorescent-based way for differentiate between healthy and apoptotic cells. It applies MitoCapture, a cationic dye that show different fluorescent-red and green in healthy and apoptotic cells, respectively. MitoCapture accumulates and aggregates in the mitochondria, emitting a bright red fluorescence in healthy cells. However, in apoptotic cells, MitoCapture lose its ability of accumulation and aggregation due to the altered mitochondrial transmembrane potential, and remain in its monomer form that emits fluorescing green.
Features & Properties
|Features||Simple one-step procedure; Takes only 30 minutes; Fast and convenient.|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
- View current batch:
|Kit components||MitoCapture Reagent, Incubation Buffer|