Proteasome Activity Fluorometric Assay Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Proteasomes are large protein complex located in nucleus and cytoplasm in all eucaryotes. They degrades and recycles the target protein that been tagged by ubiquitin. The tagged protein is hydrolyzes into 7-8 amino acids long peptides that are further degraded. The 20S assembly is the protease structure that has chymotrypsin/trypsin/caspase-like protease activities. This Proteasome Activity Assay utilized the chymotrypsin-like activity with an AMC-tagged peptide substrate that releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also contains Jurkat Cell lysate as a positive control and MG-132 as proteasome inhibitor. This can differentiate proteasome activity from other protease activities that may also in the samples.
| Features | Simple one-step procedure; Takes only 30-60 minutes; Accurate and easy to use. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
| Kit components | Proteasome Assay Buffer, Proteasome Substrate (In DMSO), Proteasome Inhibitor (In DMSO), AMC Standard (1 mM in DMSO), Positive Control |