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Proteasome Activity Fluorometric Assay Kit

Catalog No.
K2096
Specifically detects proteasome activity
Grouped product items
SizePriceStock Qty
100 assays
$390.00
In stock

Tel: +1-832-696-8203

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Background

Proteasomes are large protein complex located in nucleus and cytoplasm in all eucaryotes. They degrades and recycles the target protein that been tagged by ubiquitin. The tagged protein is hydrolyzes into 7-8 amino acids long peptides that are further degraded. The 20S assembly is the protease structure that has chymotrypsin/trypsin/caspase-like protease activities. This Proteasome Activity Assay utilized the chymotrypsin-like activity with an AMC-tagged peptide substrate that releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also contains Jurkat Cell lysate as a positive control and MG-132 as proteasome inhibitor. This can differentiate proteasome activity from other protease activities that may also in the samples.

Features & Properties

FeaturesSimple one-step procedure; takes only 30-60 minutes Accurate and easy to use
ShippingGel pack
Storage ConditionsStore at -20°C.

Quality Control

Quality Control & DataSheet

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Overview

Kit componentsProteasome Assay Buffer Proteasome Substrate (In DMSO) Proteasome Inhibitor (In DMSO) AMC Standard (1 mM in DMSO) Positive Control