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Annexin V-FITC Apoptosis Kit Plus

Catalog No.
K2057
Detects Apoptotic, Necrotic & Healthy cells within 10 min
Grouped product items
SizePriceStock Qty
25 assays
$183.00
In stock
100 assays
$422.00
In stock
400 assays
$914.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Soon after the apoptosis is activated, most cell types transfer the membrane phospholipid phosphatidylserine (PS) from the plasma membrane inner face to the cell surface. Detection of the cell-surface PS can be easily done by staining with a fluorescent conjugate of protein Annexin V which has a robust natural affinity for PS. The one-step staining process needs just 10 minute. This assay can be directly carried out on live cells without fixation. 

The Annexin V-FITC Apoptosis Detection Kit Plus includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. Following the staining the cell population with annexin V-FITC and SYROX Green dye in the given binding buffer, apoptotic cells exhibit green fluorescence, dead cells exhibits a higher level of green florescence and lives cells exhibits little or no fluorescence. 

Those cell populations can be differentiated with flow cytomery at excitation of 488 nm and emission of 530 nm.Both annexin V-FITC and SYTOX Green dye emit green fluorescence that can be detected in the FL1 channel, freeing the other channels for the addition of other probes in multi-color labeling experiments

Features & Properties

FeaturesSimple one-step procedure; Takes only 30 minutes; Fast and convenient; The Annexin VFITC Plus kit includes sytox green dye (instead of PI) which stains necrotic cells with a higher level of green fluorescence than FITC does
ShippingGel pack.
Storage ConditionsStore at +4°C.

Quality Control

Quality Control & DataSheet

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Related Biological Data

Annexin V-FITC Apoptosis Kit Plus

Related Biological Data

Annexin V-FITC Apoptosis Kit Plus

Related Biological Data

Annexin V-FITC Apoptosis Kit Plus
 

Storage

Store at +4°C.

Overview

Kit componentsAnnexin V-FITC, SYTOX Green Dye, Binding Buffer