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HAT Activity Fluorometric Assay Kit

Catalog No.
K2034
Detects HAT activity, sensitive and non-radioactive.
Grouped product items
SizePriceStock Qty
100 assays
$438.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Histone acetyltransferase (HAT) is an enzyme that acetylates conserved lysine on a histone protein by transferring an acetyl group from acetyl CoA. Histone acetylation can increase gene expression. HAT plays important roles in gene transcription, cell proliferation and differentiation.

 The HAT Activity Fluorometric Assay Kit provides a sensitive and sensitive way for detection of HAT activity in a variety of samples based on fluorometric method that eliminates radioactivity in traditional assays. The assay utilizes H3 histone peptide and Acetyl CoA as substrates. The active HAT transfers acetyl groups from Acetyl-CoA to the H3 histone peptide, thereby producing two products - acetylated H3 histone peptide and CoA-SH. The CoA-SH reacts with the developer to yield a fluorophore product that can be easily detected using a fluorometer or a fluorescence plate reader at Ex/Em = 535/587 nm. The kit can determine HAT activity as low as 6 mU in a variety of samples.

Features & Properties

FeaturesSimple, rapid and convenient. The assay can detect HAT Activity as low as 6 mU in a variety of samples.
ShippingDry ice
Storage ConditionsStore at -80°C.

Quality Control

Quality Control & DataSheet

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Related Biological Data

HAT Activity Fluorometric Assay Kit

Related Biological Data

HAT Activity Fluorometric Assay Kit

Related Biological Data

HAT Activity Fluorometric Assay Kit
 

Overview

Kit componentsHAT Assay Buffer Acetyl CoA (Lyophilized) H3 Peptide (Lyophilized) Substrate Mix (Lyophilized) Developer PicoProbe™ CoA Standard (Lyophilized) Positive Control (HeLa Nuclear Extract)