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HAT Activity Colorimetric Assay Kit

Catalog No.
K2033
Detects HAT activity, sensitive and non-radioactive.
Grouped product items
SizePriceStock Qty
100 assays
$438.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Histone acetyltransferase (HAT) is an enzyme that acetylates conserved lysine on a histone protein by transferring an acetyl group from acetyl CoA. Histone acetylation can increase gene expression. HAT plays important roles in gene transcription, cell proliferation and differentiation.

 The HAT Activity Colorimetric Assay Kit provides a fast and sensitive way for detection of HAT activity in mammalian samples based on colorimetric method that eliminates radioactivity in traditional assays. The assay utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. The active HAT acetylates peptide substrate and then releases the free form of CoA which then acts as an essential coenzyme for generating NADH. NADH can be easily detected upon reacting with a soluble tetrazolium dye using a spectrophotometer or an ELISA plate reader. The detection can be continuous and suited for kinetic studies.

Features & Properties

FeaturesFast and convenient. Simple procedure The detection can be continuous and suitable for kinetic studies.
ShippingDry ice
Storage ConditionsStore at -80°C.

Quality Control

Quality Control & DataSheet

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Related Biological Data

HAT Activity Colorimetric Assay Kit

Related Biological Data

HAT Activity Colorimetric Assay Kit

Related Biological Data

HAT Activity Colorimetric Assay Kit

Related Biological Data

HAT Activity Colorimetric Assay Kit
 

Overview

Kit components2X HAT Assay Buffer HAT Substrate I HAT Substrate II NADH Generating Enzyme HAT Reconstitution Buffer Nuclear Extract 4 mg/mL