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Trichostatin A (TSA)

In stock
Catalog No.
A8183
HDAC inhibitor
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$210.00
In stock
1mg
$60.00
In stock
5mg
$190.00
In stock
25mg
$700.00
In stock
100mg
$1,800.00
In stock

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Trichostatin A (TSA) is a potent inhibitor of histone deacetylase (HDAC) as well as an antifungal antibiotic with cytostatic and differentiating properties that noncompetivively and reversibly inhibits HDAC, at low nanomolar concentrations, in both cultured mammalian cells and fractionated cell nuclear extracts. It is capable of arresting cells in G1 and G2 phases of the cell cycle, inducing differentiation and reverting the transformed morphology of cells in culture. According to a study investigating the effect of TSA in human breast cancer cell lines, TSA inhibited proliferation of breast carcinoma cell lines (IC50 124.4 ± 120.4 nM), comparing to all cell lines (IC50 2.4 ± 0.5 nM), and resulted in pronounced histone H4 hyperacetylation.

Reference

David M. Vigushin, Simak Ali, Paul E. Pace, Nina Mirsaidi, Kazuhiro Ito, Ian Adcock, and R. Charles Coombes. Trichostatin A is a histone deacetylase inhibitor with potent antitumot activity against breast cancer in vivo. Clin Cancer Res 2001;7:971-976

Product Citation

Chemical Properties

StorageDesiccate at -20°C
M.Wt302.37
Cas No.58880-19-6
FormulaC17H22N2O3
SynonymsTrichostatin A,TSA
Solubility≥15.12mg/mL in DMSO
Chemical Name(2E,4E,6R)-7-[4-(dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxohepta-2,4-dienamide
SDFDownload SDF
Canonical SMILESCC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell experiment[1]:

Cell lines

Human breast cancer cell line

Preparation method

The solubility of this compound in DMSO is <10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Reaction Conditions

10 μM TSA solved in growth medium containing 0.1% (v/v) ethanol for 96 h incubation

Applications

TSA inhibited proliferation of eight breast carcinoma cell lines with mean ± SD IC50 of 124.4 ± 120.4 nM (range, 26.4–308.1 nM). TSA treatment resulted in pronounced histone H4 hyperacetylation.

Animal experiment [1]:

Animal models

Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU

Dosage form

500 μg/kg by injection daily for 4 weeks

Applications

TSA had pronounced antitumor activity in vivo. that The antitumor activity of TSA attributabled to induction of differentiation.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

1. Vigushin DM1, Ali S, Pace PE et al. Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin Cancer Res. 2001 Apr;7(4):971-6.

Biological Activity

Targets HDAC          
IC50 ~1.8 nM          

Quality Control

Quality Control & MSDS

View current batch:

Chemical structure

Trichostatin A (TSA)

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