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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
T4 DNA Ligase is a single-stranded polypeptide with a molecular weight of 55.3 kDa, which can catalyze the formation of phosphodiester bonds at adjacent 5’ phosphate and 3’ hydroxyl ends on double-stranded DNA or RNA. The ligase has no catalytic activity for single-stranded nucleic acids, and its catalytic reaction requires ATP as a cofactor.
T4 DNA ligase is versatile, can not only bind DNA fragments to cohesive or blunt ends, but also repair single-stranded nicks on double-stranded DNA, double-stranded RNA, or DNA/RNA hybrids. It is commonly used for connection of DNA fragments with vectors, linkers or adaptors; nicks repair; and linear DNA self-cyclization.
Store the components at -20 °C.