Toggle Nav
Close
  • Menu
  • Setting

SYBR Safe DNA Gel Stain

Catalog No.
A8743
Safe and sensitive DNA/RNA gel stain
Grouped product items
SizePriceStock Qty
500ul
$60.00
In stock
1ml
$96.00
In stock
10x1ml
$560.00
In stock
100x1ml
$2,880.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. It is specifically formulated to be a less hazardous alternative to ethidium bromide (EB) that can be used with either blue-light or UV excitation.

SYBR Safe DNA Gel Stain is a better nucleic acid staining reagent for all your molecular biology research needs. It is not only environmental friendly but also good for your sample.

SYBR Safe DNA Gel Stain is supplied as 10000X concentrate in DMSO and used in the same way as ethidium bromide solution. It is also suitable for staining RNA in gels.

Cloning efficiency of DNA fragments is improved by using SYBR Safe DNA Gel Stain and blue-light, compared with using ethidium bromide and UV light exposure.

Product Citation

Features

• Reduce exposure to highly mutagenic EB and harmful UV light.
SYBR Safe DNA Gel Stain is less mutagenic and safer for you to work with than ethidium bromide. You can further decrease your exposure risk by using visible blue-light with SYBR Safe stain rather than UV. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

• Increase sensitivity by reducing nonspecific background fluorescence.
SYBR Safe DNA Gel Stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. Plus, when used with blue light, SYBR Safe DNA Gel Stain has less background fluorescence than ethidium bromide–stained gels with UV light.

• Alternative to EB for all staining applications, including RNA staining.
SYBR Safe DNA Gel Stain is supplied as a concentrate in DMSO which can be used in the same way as ethidium bromide. It can be added into an agarose gel for staining during electrophoresis at a ratio of 1: 10000 or apply it after electrophoresis at a ratio of 1: 3300. Store at room temperature in its original packaging to avoid excessive light exposure.

Note: SYBR Safe DNA Gel Stain is less efficient in visualizing low molecule weight bands (100-200 bp).

Chemical Properties

Physical AppearanceDMSO solution
StorageStore at room temperature for use. And store at -20°C for long-term storage
M.Wt504.66
FormulaC28H28N2O3S2
Solubilityinsoluble in EtOH; insoluble in H2O; ≥14.67 mg/mL in DMSO
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Related Biological Data

SYBR Safe DNA Gel Stain
The agarose gel using SYBR Safe DNA Gel Stain (1:10000 dilute). The volume of loading samples is 5 ul, 10 ul and 15 ul, respectively.

Related Biological Data

SYBR Safe DNA Gel Stain

Related Biological Data

SYBR Safe DNA Gel Stain

Related Biological Data

SYBR Safe DNA Gel Stain