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Sulforaphane

Catalog No.
C4733
inducer of chemopreventative enzymes via Keap1-Nrf2 signaling
Grouped product items
SizePriceStock Qty
10mg
$60.00
In stock
25mg
$135.00
In stock
50mg
$250.00
In stock

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Email: [email protected]

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Background

Sulforaphane is an inducer of chemopreventative enzymes via Keap1-Nrf2 signaling [1]. Sulforaphane is present naturally in widely consumed vegetables and has a particularly high concentration in broc-coli [1].

The Keap1-Nrf2 pathway is the major regulator of cytoprotective responses to oxidative and electrophilic stress. Nrf2 triggers the signaling pathways to prevent cancer initiation and progression in normal and premalignant tissues. In fully malignant cells, Nrf2 activity provides growth advantage by increasing cancer chemoresistance and enhancing tumor cell growth [2].

In human colon carcinoma cells (HT29) cells, sulforaphane at 15 μM inhibited cell growth and induced cell death. Sulforaphane induced a cell cycle arrest in a dose-dependent manner, followed by cell death. Sulforaphane induced cell cycle arrested in G2 -M Phase, which was correlated with increased expression of cyclins A and B1. Sulforaphane increased expression of the proapoptotic protein bax, the release of cytochrome c from the mitochondria to the cytosol, and the proteolytic cleavage of poly(ADP-ribose) polymerase [1].

References:
[1] Gamet-Payrastre L, Li P, Lumeau S, et al.  Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells[J]. Cancer research, 2000, 60(5): 1426-1433.
[2] Kansanen E, Kuosmanen S M, Leinonen H, et al.  The Keap1-Nrf2 pathway: mechanisms of activation and dysregulation in cancer[J]. Redox biology, 2013, 1(1): 45-49.

Product Citation

Chemical Properties

StorageStore at -20°C, Light exposure should be avoided
M.Wt177.3
Cas No.4478-93-7
FormulaC6H11NOS2
SynonymsSFN
Solubility≥51.6 mg/mL in H2O; ≥58.2 mg/mL in EtOH; ≥67.6 mg/mL in DMSO
Chemical Name1-isothiocyanato-4-(methylsulfinyl)-butane
SDFDownload SDF
Canonical SMILESCS(CCCCN=C=S)=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

HT29 human colon cancer cells

Reaction Conditions

0 ~ 30 μM sulforaphane for 48 h incubation

Applications

Sulforaphane resulted in a net decrease in the total number of cells and an accumulation of cells floating in the culture medium. The inhibition of growth and the effect on the adherence were already important at 15 μM. Sulforaphane at 15 μM was able to inhibit cell growth and induce cell death. Cell mortality was already 75% at 24 h and almost total at 96 h.

Animal experiment:[2]

Animal models

Female Sprague-Dawley rats, 40 days of age

Dosage form

75 or 150 μmol

Once daily by gavage for 5 days

Applications

When sulforaphane was administered by gavage (75 or 150 μmol per day for 5 days) around the time of exposure to the 9,10-dimethyl-1,2-benzanthracene carcinogen, the incidence, multiplicity, and weight of mammary tumors were significantly reduced, and their development was delayed. Sulforaphane was able to protect against carcinogenesis.

Note

The technical data provided above is for reference only.

References:

1. Gamet-Payrastre L, Li P, Lumeau S, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Research, 2000, 60(5): 1426-1433.

2. Zhang Y, Kensler TW, Cho CG, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothiocyanates. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(8): 3147-3150.

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