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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
M-1 cells
Preparation method
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.
Reacting condition
1 nM ~ 1 μM; 24 hrs
Applications
In M-1 cells, Medroxyprogesterone Acetate at the dose of 1 μM increased α-epithelial Na channel (α-ENaC) and serum and glucocorticoid-regulated kinase 1 (sgk1) expression. Medroxyprogesterone Acetate dose-dependently increased α-ENaC expression with the earliest effect seen at 10 nM. In Medroxyprogesterone Acetate-treated M-1 cells, α-ENaC-driven luciferase activity could not be inhibited by Org31710, which indicated that Medroxyprogesterone Acetate regulated α-ENaC in a progesterone receptor (PR)-independent manner.
Animal models
Rats
Dosage form
14 and 21 mg/2 mL; given via osmotic pumps
In aged ovariectomized rats, Medroxyprogesterone acetate impaired delayed memory retention on the water radial-arm maze, and exacerbated overnight forgetting on the Morris maze. Medroxyprogesterone acetate significantly decreased the level of glutamic acid decarboxylase (GAD) in the hippocampus, and increased GAD level in the entorhinal cortex.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Thomas CP, Liu KZ, Vats HS. Medroxyprogesterone acetate binds the glucocorticoid receptor to stimulate alpha-ENaC and sgk1 expression in renal collecting duct epithelia. Am J Physiol Renal Physiol. 2006 Feb;290(2):F306-12. Epub 2005 Sep 27.
[2]. Braden BB, Talboom JS, Crain ID, Simard AR, Lukas RJ, Prokai L, Scheldrup MR, Bowman BL, Bimonte-Nelson HA. Medroxyprogesterone acetate impairs memory and alters the GABAergic system in aged surgically menopausal rats. Neurobiol Learn Mem. 2010 Mar;93(3):444-53.