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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Antibiotic and antitumor agent. Alkylates DNA and induces diabetes mellitus via reduction of nicotinamide adenine dinucleotide in pancreatic β-cells in vivo.
Cell lines
A murine pancreatic β cell line, INS-1
Reaction Conditions
15 or 30 mM streptozocin for 1 h incubation
Applications
Higher rates of apoptosis, as compared to necrosis, were observed when cells were exposed to 15 mM streptozocin for 1 h followed by a 24 h recovery period. Higher doses of streptozocin (30 mM) caused the cells to undergo necrosis (22%) as well as apoptosis (17%). Streptozotocin at low doses induced apoptosis and at high doses caused necrosis in INS-1 cells.
Animal models
Male adult Holtzman rats
Dosage form
50, 65 or 100 mg/kg
A single intravenous injection
Streptozotocin (65 mg/kg) resulted in rapid degranulation of β cells, accumulation of glycogen in the proximal convoluted tubules of the kidney, as well as development of cataracts in 4 months after streptozotocin treatment. At a higher dose of 100 mg/kg, streptozotocin produced lesions in the exocrine cells of the pancreas, and led to persistence of small, possibly secretory, granules in the Golgi zone of β cells in diabetic rats. Streptozotocin is often used to induce diabetes mellitus in experimental animals.
Note
The technical data provided above is for reference only.
References:
1. Saini KS, Thompson C, Winterford CM, et al. Streptozotocin at low doses induces apoptosis and at high doses causes necrosis in a murine pancreatic beta cell line, INS-1. Biochemistry and Molecular Biology International, 1996, 39(6): 1229-1236.
2. Arison RN, Ciaccio EI, Glitzer MS, et al. Light and electron microscopy of lesions in rats rendered diabetic with streptozotocin. Diabetes, 1967, 16(1): 51-56.