JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Tel: +1-832-696-8203
Email: [email protected]
Worldwide Distributors
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cells treatment with the cytotoxic compound may undergo necrosis, in which they lose membrane integrity. Hence cytotoxicity can be detected by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme exists in nearly all living cells. When damage of the plasma membrane, LDH is rapidly released into the cell culture supernatant.
The LDH-Cytotoxicity Colorimetric Assay Kit provides a simple and fast way for the quantification of cytotoxicity based on the measurement of activity of LDH released from damaged cells, which can be detected by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which reacts with tetrazolium salt INT to form formazan. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The assay is convenient, precise and sensitive, and is suitable for a variety of cytotoxicity studies and only takes 0.5-1 h.