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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Phalloidin, a mushroom-derived toxin, can be used to label cytoskeletal F-actin with fluorochrome (λex = 495 nm, λem = 520 nm). Also, phalloidin can react stoichiometrically with muscle actin, substantially promote actin polymerization, and stabilize polymerized actin. Actin is the major structural protein of the cytoplasm of eukaryotic cells, and a well-balanced equilibrium between different polymerization states of actin is important for various cellular functions such as cell locomotion and cell growth.
References:
1. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947.
2. Wehland J, Osborn M, Weber K. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proceedings of the National Academy of Sciences of the United States of America, 1977, 74(12): 5613-5617.
Cell lines
Mouse 3T3 and rat kangaroo PtK2 cells
Reaction Conditions
0.2 or 1 mM phalloidin in 0.14 M KCI (Me2SO concentration 0.4 or 2%), for 3 h incubation
Applications
When phalloidin was injected into cells, it recruited the less highly polymerized forms of actin and/or G actin, to form "islands" of aggregated actin in a focal plane above the stress fibers. In addition, microinjection of phalloidin interfered cell locomotion and cell growth in a concentration-dependent manner.
Note
The technical data provided above is for reference only.