In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB590885 is a potent and selective inhibitor of B-Raf kinase with Ki value of 0.16nM .
SB590885 is a potent inhibitor of oncogenic B-Raf protein kinase with Ki value of 0.16nM. It is more potent to inhibit B-Raf than C-Raf. The Ki value of SB590885 for C-Raf is 1.72nM. SB590885 is a quite selective inhibitor. It shows no activity against 48 other human kinases such as Abl, AMPK, CK1, CK2 and ERK2. It is found that SB590885 binds to B-Raf within the ATP-binding pocket and stabilizes the active conformation of B-Raf. SB590885 decreases the phosphorylation of ERK and shows anti-proliferation only in tumor cells expressing oncogenic B-Raf V600E. The normal cells and tumor cells not expressing mutant B-Raf have no sensitivity towards SB590885 except the normal melanocytes and primary melanoma cells expressing wild-type B-Raf. Moreover, SB590885 is also found to decrease the transformed and tumorigenic properties of malignant cells expressing mutant B-Raf .
 King A J, Patrick D R, Batorsky R S, et al. Demonstration of a genetic therapeutic index for tumors expressing oncogenic BRAF by the kinase inhibitor SB-590885. Cancer research, 2006, 66(23): 11100-11105.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥4.54mg/mL in DMSO with gentle warming|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Description||SB590885 is a potent inhibitor of B-Raf with a Ki value of 0.16 nM.|