Home >> Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Related Products
Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)
Equivalent to Sigma P8849, for use in purification of Histidine-tagged proteins to increase protein stability.

Catalog No.K1010
Size Price Stock Qty
1ml
$35.00
In stock
10x1ml
$250.00
In stock
50x1ml
$1,000.00
In stock
100x1ml
$1,500.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

      

Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & DataSheet

View current batch:

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and storage

Catalog No. Product Name Summary Targets CAS Number Smiles
A2573 AEBSF.HCl Serine protease inhibitor Proteases|Serine Protease 30827-99-7 C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2575 Bestatin Aminopeptidase inhibitor Proteases|Aminopeptidase 58970-76-6 CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576 E-64 Cysteine protease inhibitor,irriversible Proteases|Cathepsin 66701-25-5 CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 26305-03-3 CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
B4790 Phosphoramidon Disodium Salt metalloproteinase inhibitor 164204-38-0 CC(C[[email protected]@](NP(O)(O[[email protected]@]1([H])[[email protected]@](O)([H])[[email protected]@](O)([H])[[email protected]](O)([H])[[email protected]](O1)([H])C)=O)([H])/C([O-])=N/[[email protected]@](C([O-])=O)([H])CC2=CNC3=CC=CC=C23)C.[Na+].[Na+]
Stored at -20°C, and stable for at least 12 months.

Description

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in purification of Histidine-tagged proteins to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

This protease inhibitor cocktail contains individual components, including AEBSF, Bestatin, E-64, Phosphoramidon and Pepstatin A with a broad specificity for cysteine, serine, aspartic, and thermolysin-like proteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for purification of histidine-tagged proteins. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Advantage

Accurate Ingredients:

Clearance of components and concentrations.


Higher efficiency:

Quick achievement, quality assurance.


Lower price:

Our price is less than any of competitor.