In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ONO-8711 is a novel and selective EP1 antagonist.
Prostaglandin (PG) E2 functions by acting on a group of G-protein-coupled receptors (GPCRs) EP receptros. The EP receptors exhibit differences in signal transduction, tissue localization, and regulation of expression. The EP receptors have been implicated in various physiological and pathophysiological functions .
In vitro: In human pulmonary vessels, ONO-8711 (30 μM) non-competitively blocked the contractions produced with sulprostone, an EP1,3 agonist .
In vivo: In azoxymethane-treated C57BL/6J mice, administration of 250, 500, or 1000 ppm of ONO-8711 dose-dependently reduced ACF formation. In Min mice with a nonsense mutation in the adenomatous polyposis coli gene, treatment with 500 ppm ONO-8711 significantly reduced the number of intestinal polyps . In female SD rats given PhIP, administration of ONO-8711 at 400 or 800 p.p.m. delayed occurrence of breast tumors for 2 or 4 weeks, respectively . Treatment with ONO-8711 (800 p.p.m.) significantly decreased PhIP-induced breast cancer incidence, multiplicity and volume when compared with those of control rats .
 Watanabe K, Kawamori T, Nakatsugi S, et al. Role of the prostaglandin E receptor subtype EP1 in colon carcinogenesis[J]. Cancer research, 1999, 59(20): 5093-5096.
 Sugimoto Y, Narumiya S. Prostaglandin E receptors[J]. Journal of Biological Chemistry, 2007, 282(16): 11613-11617.
 Norel X, de Montpreville V, Brink C. Vasoconstriction induced by activation of EP 1 and EP 3 receptors in human lung: effects of ONO-AE-248, ONO-DI-004, ONO-8711 or ONO-8713[J]. Prostaglandins & other lipid mediators, 2004, 74(1): 101-112.
 Kawamori T, Uchiya N, Nakatsugi S, et al. Chemopreventive effects of ONO-8711, a selective prostaglandin E receptor EP1 antagonist, on breast cancer development[J]. Carcinogenesis, 2001, 22(12).
|Physical Appearance||A solution in methyl acetate|
|Storage||Store at -20°C|
|Solubility||≤20mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide|
|Chemical Name||6-[(2R,3S)-3-[[[(4-chloro-2-methylphenyl)sulfonyl]amino]methyl]bicyclo[2.2.2]oct-2-yl]-5Z-hexenoic acid|
|Canonical SMILES||ClC1=CC(C)=C(S(N([H])C[[email protected]@H](C2CCC3CC2)[[email protected]]3/C=C\CCCC(O)=O)(=O)=O)C=C1|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|