In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 3852 is a potent inhibitor of histone deacetylase .
Histone deacetylases (HADC) are a series of enzymes that remove acetyl groups from an ε-N-acetyl lysine amino acid on a histone and make the histones to wrap the DNA more tightly, which prevent transcription.
In MCF-7 human breast cancer cells, NSC 3852 induced the formation of the DMPO-OH adduct, which was catalyzed by superoxide dismutase. The result suggested that NSC 3852 induced generation of superoxide and reactive oxygen species (ROS), which produced DNA damage and induced apoptosis. Also, NSC 3852 resulted in cells accumulation in G0, hypophosphorylated retinoblastoma protein(Rb) and reduced E2F1 and Myc protein . In HS68 cell, NSC 3852 inhibited Toxoplasma gondii tachyzoite propagation with EC50 value of 78.6 nM. Also, in human red blood cells, NSC 3852 inhibited Plasmodium falciparum growth with EC50 value of 1.3 μM. NSC 3852 inhibited Toxoplasma gondii tachyzoite propagation by involving redox reactions .
. Martirosyan A, Leonard S, Shi X, et al. Actions of a histone deacetylase inhibitor NSC3852 (5-nitroso-8-quinolinol) link reactive oxygen species to cell differentiation and apoptosis in MCF-7 human mammary tumor cells. J Pharmacol Exp Ther, 2006, 317(2): 546-552.
. Strobl JS, Seibert CW, Li Y, et al. Inhibition of Toxoplasma gondii and Plasmodium falciparum infections in vitro by NSC3852, a redox active antiproliferative and tumor cell differentiation agent. J Parasitol, 2009, 95(1): 215-223.
|Physical Appearance||A solid|
|Storage||Store at RT|
|Solubility||≥7.35mg/mL in DMSO, <2.06 mg/mL in EtOH, <2.52 mg/mL in H2O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|