In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Niclosamide is an inhibitor of the signal transducer and activator of transcription 3 (STAT3) with IC50 value of 0.7 μM .
STATs are a class of transcription factors that regulate fundamental cellular and biological processes, such as cell proliferation, cell survival, immune responses, and angiogenesis, by modulating the expression of specific target genes. STAT3 has been frequently reported to be over-activated in various human cancer types .
In HeLa cells, Niclosamide (< 5 μM) inhibited STAT3-mediated luciferase reporter activity with in a dose dependent manner, with IC50 value of 0.25 μM. In Du145 cancer cells, Niclosamide(< 10 μM) dose-dependently induced G0/G1 arrest and apoptosis .
In nude mice bearing HL-60 xenograft tumors, Niclosamide treatment (40 mg/kg/d, i.p.) for 15 days potently inhibited the growth of HL-60 tumors. Further, immunoblotting of xenograft tissues from mice revealed a potent inhibitory effect of Niclosamide on the pathway of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) .
. Ren X, Duan L, He Q, et al. Identification of Niclosamide as a New Small-Molecule Inhibitor of the STAT3 Signaling Pathway. ACS Medicinal Chemistry Letters, 2010, 1(9): 454-459.
. Jin Y, Lu Z, Ding K, et al. Antineoplastic mechanisms of niclosamide in acute myelogenous leukemia stem cells: inactivation of the NF-kappaB pathway and generation of reactive oxygen species. Cancer Research, 2010, 70(6): 2516-2527.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥12.75mg/mL in EtOH with gentle warming|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
Quality Control & MSDS
- View current batch:
Purity = 99.76%
- COA (Certificate Of Analysis)
- HPLC (Retest)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)