endogenous nitric oxide synthase inhibitor
Sample solution is provided at 25 µL, 10mM.
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|Cas No.||220805-22-1||SDF||Download SDF|
|Chemical Name||N5-[(dimethylamino)iminomethyl]-L-ornithine, dihydrochloride|
|Canonical SMILES||OC(=O)[[email protected]@H](N)CCCNC(=N)N(C)C|
|Formula||C8H18N4O2 • 2HCl||M.Wt||275.2|
|Solubility||≤3.5mg/ml in ethanol;3.5mg/ml in DMSO;3.5mg/ml in dimethyl formamide||Storage||Store at -20°C|
|Physical Appearance||A crystalline solid||Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
NG,NG-dimethyl-L-Arginine is an endogenous nitric oxide synthase inhibitor.
Nitric oxide (NO) that is synthesised from L-arginine contributes to the regulation of blood pressure and to host defence.
In vitro: NG,NG-dimethyl-L-Arginine (ADMA) was found to be able to inhibit macrophage and vascular NO synthase. When added to a cytosolic preparation of macrophage NO synthase, ADMA could cause dose-dependent inhibition of NO formation. In the presence of added arginine, ADMA could inhibited NO synthesis and the inhibitory effects of ADMA were prevented by addition of L-arginine so that the rate of NO synthesis did not significantly differ from control values .
In vivo: Animal study showed that the injection of 1-30 mg/kg ADMA into 3 anaesthetised guineapigs could cause dose-dependent increases in mean arterial blood pressure. Moreover, during continuous infusion of ADMA, which caused plasma ADMA concentrations to rise approximately 9-fold, the systolic blood pressure was raised by nearly 15 % .
Clinical trial: Local infusion of ADMA into the brachial artery of healthy volunteers caused a dose-dependent fall in forearm blood-flow. Infusion of ADMA for 5 min resulted in a fall in forearm blood-flow in 5 subjects, and the effects of this ADMA dose were significantly attenuated by infusion of L-arginine .
 Vallance, P. ,Leone, A.,Calver, A., et al. Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 339, 572-575 (1992).