In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NG,NG-dimethyl-L-Arginine is an endogenous nitric oxide synthase inhibitor.
Nitric oxide (NO) that is synthesised from L-arginine contributes to the regulation of blood pressure and to host defence.
In vitro: NG,NG-dimethyl-L-Arginine (ADMA) was found to be able to inhibit macrophage and vascular NO synthase. When added to a cytosolic preparation of macrophage NO synthase, ADMA could cause dose-dependent inhibition of NO formation. In the presence of added arginine, ADMA could inhibited NO synthesis and the inhibitory effects of ADMA were prevented by addition of L-arginine so that the rate of NO synthesis did not significantly differ from control values .
In vivo: Animal study showed that the injection of 1-30 mg/kg ADMA into 3 anaesthetised guineapigs could cause dose-dependent increases in mean arterial blood pressure. Moreover, during continuous infusion of ADMA, which caused plasma ADMA concentrations to rise approximately 9-fold, the systolic blood pressure was raised by nearly 15 % .
Clinical trial: Local infusion of ADMA into the brachial artery of healthy volunteers caused a dose-dependent fall in forearm blood-flow. Infusion of ADMA for 5 min resulted in a fall in forearm blood-flow in 5 subjects, and the effects of this ADMA dose were significantly attenuated by infusion of L-arginine .
 Vallance, P. ,Leone, A.,Calver, A., et al. Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 339, 572-575 (1992).
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤3.5mg/ml in ethanol;3.5mg/ml in DMSO;3.5mg/ml in dimethyl formamide|
|Chemical Name||N5-[(dimethylamino)iminomethyl]-L-ornithine, dihydrochloride|
|Canonical SMILES||OC(=O)[[email protected]@H](N)CCCNC(=N)N(C)C|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|