Live-Dead Cell Staining Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Our product Live-Dead Cell Staining Kit is used for simultaneous fluorescence staining of viable and dead cells. This kit contains two dyes-Calcein-AM and Propidium Iodide (PI), which stain viable and dead cells, respectively.
Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though Calcein-AM itself isn’t a fluorescent molecule, it will turn to Calcein after esterase’s digestion in a viable cell, which could emit strong green fluorescence (excitation: 490 nm, emission: 515 nm). Therefore, Calcein-AM only stains viable cells.
In contrast, PI, a nuclei staining dye, can’t pass through a viable cell membrane. It reaches the nucleus by passing through disorderedareas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (excitation: 535 nm, emmision: 617 nm).
Since both calcein and PI-DNA can be excited with 490 nm, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope or flow cytometer. With 545 nm excitation, only dead cells can emit red fluorescence. Since optimal staining conditions constantly changes because of different cell lines, we recommend that a suitable concentration of PI and Calcein-AM should be individually determined.
Please note that PI is suspected to be highly carcinogenic; careful handling is required.
Quality Control & DataSheet
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Components and Storage
|500 Tests||1000 Tests|
|Calcein-AM Solution (2 mM)||50 μL||50 μL × 2|
|PI Solution (1.5 mM)||150 μL||150 μL × 2|
Store at -20°C, protect from light.
Calcein-AM is easily hydrolized by moisture. Keep it desiccate and tightly close the cap after the use.