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Catalog No.
JNK inhibitor, selective and irreversible
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
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In stock
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In stock

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JNK-IN-8 is a specific JNK1/2/3 inhibitor with IC50 value of 4.67, 18.7, 0.98 nM respectively [1].

C-Jun N-terminal kinase (JNK) 1, 2 and 3 belong to the mitogen-activated protein kinase (MAPK) family, which are able to phosphorylate c-Jun on the Ser63 and Ser73 residue.They are responsive for stress stimuli, including cytokines and heat shock, and get involved in T cell differentiation and cell apoptosis process. JNK 1 and 2 are ubiquitous in all cell types but JNK 3 is only found in cells of brain, heart and testes tissues.

JNK-IN-8 is a JNK1/2/3 inhibitor with high specificity. When JNK-IN-8 was profiled with a panel of 400 kinases, it exhibited specific binding to JNK 1/2/3 but not to other kinases. Crystallization study also found that JNK-IN-8 forms covalent bonds with conserved cysteine residue of JNK 1/2/3, resulting in a conformational change of the activation loop that blocks the substrate binding, thereby inhibiting the activity of JNK 1/2/3 [1].

In Hela cells and A375 cells, pretreatment of cells with JNK-IN-8 resulted in the inhibition of c-Jun which is a direct phosphorylation substrate of JNK 1/2/3, confirming the inhibitory action of JNK-IN-8 on JNK 1/2/3. In HEK293-ILR1 cells following stimulation by anisomycin, the JNK-IN-8 was observed to inhibit c-Jun but not MSK1 and p38, and the inhibition was not reversible by removing JNK-IN-8 from culture medium. Additionally, JNK-IN-8 only exhibited on-pathway inhibition of JNK signaling pathway, which can be monitored by the phosphorylation of c-Jun [1].

[1].  Zhang T et al., Discovery of potent and selective covalent inhibitors of JNK. Chemical Biology. 2012, 19(1):140-154.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
Cas No.1410880-22-6
Solubility≥25.4 mg/mL in DMSO; insoluble in H2O; ≥9.24 mg/mL in EtOH with gentle warming and ultrasonic
Chemical Name3-[[(E)-4-(dimethylamino)but-2-enoyl]amino]-N-[3-methyl-4-[(4-pyridin-3-ylpyrimidin-2-yl)amino]phenyl]benzamide
SDFDownload SDF
Canonical SMILESCC1=C(C=CC(=C1)NC(=O)C2=CC(=CC=C2)NC(=O)C=CCN(C)C)NC3=NC=CC(=N3)C4=CN=CC=C4
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.


Cell experiment [1]:

Cell lines

HEK293-ILR1 cells

Preparation method

Soluble in DMSO > 25.4mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.1,0.3,1,3μM for 3hr


JNK-IN-8 was an extremely potent inhibitor of enzymatic and cellular JNK(c-Jun N-terminal kinase) that inhibited phosphorylation of c-Jun, the direct substrate of JNK kinase. The superior potency and selectivity of JNK-IN-8 in the HEK293 cells suggested that the compound would likely serve as very useful pharmacological probes of JNK-dependent cellular phenomena.

Animal experiment [2]:

Animal models

Male KM mice (CL)(8-week-old)

Dosage form

3μg/μL, injection


JNK-IN-8, a specific inhibitor of JNK pathway, could reduce the neuronal apoptosis significantly as compared to the DMSO group after brain injury in the mice.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.


[1]. Zhang T1, Inesta-Vaquera F, et al, Discovery of potent and selective covalent inhibitors of JNK. Chemical Biology. 2012, 19(1):140-154.

[2]. Li D1, Liu N1,et al, Protective effect of resveratrol against nigrostriatal pathway injury in striatum via JNK pathway. Brain Res. 2017 Jan 1;1654(Pt A):1-8. doi: 10.1016/j.brainres.2016.10.013. Epub 2016 Oct 18.

Biological Activity

Description JNK-IN-8 is a selective and irreversible type 2 inhibitor of JNK with IC50 value of 4.67 nM, 18.7 nM and 980 pM for JNK1, JNK2 and JNK3, respectively.
Targets JNK1 JNK2 JNK3      
IC50 4.67 nM 18.7 nM 980 pM      

Quality Control

Chemical structure