Ionomycin calcium salt
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ionomycin calcium salt is a calcium ionophore .
Ca2+ ionophore acts as a Ca2+ ionophore, allowing Ca2+ to cross cell membranes.
Ionomycin calcium salt is a calcium ionophore. In cultured chicken pectoralis muscle, ionomycin (10-6-10-5 M) selectively increased the incorporation of methionine into two proteins. Ionomycin increased influx of extracellular Ca2+ and increased Ca2+ concentration in the cytoplasm, which then regulated the synthesis of muscle proteins . In rat parotid gland, ionomycin increased 86Rb efflux, 22Na uptake and 3H-protein secretion, which were dependent on increasing cytosolic Ca2+. In parotid cells, ionomycin released the receptor-regulated cellular Ca2+ pool . In human bladder cancer cells HT1376, ionomycin significantly inhibited cell growth in a time- and dose-dependent way and induced apoptotic DNA degradation. Also, ionomycin reduced the ratios of Bcl-2 to Bax mRNA and protein .
In athymic nude mice bearing HT1376 tumors, ionomycin significantly inhibited tumor growth and reduced the tumorigenicity .
. Liu C, Hermann TE. Characterization of ionomycin as a calcium ionophore. J Biol Chem, 1978, 253(17): 5892-5894.
. Roufa D, Wu FS, Martonosi AN. The effect of Ca2+ ionophores upon the synthesis of proteins in cultured skeletal muscle. Biochim Biophys Acta, 1981, 674(2): 225-237.
. Poggioli J, Leslie BA, McKinney JS, et al. Actions of ionomycin in rat parotid gland. J Pharmacol Exp Ther, 1982, 221(1): 247-253.
. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. J Urol, 1999, 162(3 Pt 1): 916-921.
|Physical Appearance||A crystalline solid|
|Storage||Desiccate at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||calcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate|
|Canonical SMILES||O[[email protected]](C)[[email protected]@]1(C)O[[email protected]](CC1)[[email protected]]2(C)O[[email protected]@H](C[[email protected]@H]([[email protected]@H](C)[[email protected]]([[email protected]](/C=C/C[[email protected]@H](C)C[[email protected]@H](C)/C([O-])=C\C([[email protected]@H](C)C[[email protected]@H](C)C[[email protected]@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2]|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|