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ID-8 DYRK inhibitor

Catalog No.B1952
Size Price Stock Qty
10mM (in 1mL DMSO)
$50.00
In stock
10mg
$68.00
In stock
50mg
$259.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Sample solution is provided at 25 µL, 10mM.

Quality Control

Chemical structure

ID-8

Protocol

Cell experiment [1]:

Cell lines

D3ES embryonic stem cells (ESCs)

Preparation method

The solubility of this compound in DMSO is >13.95mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

10 μM; more than 1 month

Applications

In D3ES embryonic stem cells (ESCs) cultured in serum-free media, ID-8 enabled ESCs to proliferate in the undifferentiated state for at least 30 d. Cells maintained in the medium supplemented with ID-8 differentiated into various cell types, such as neurons, muscles, and liver. Removal of ID-8 from the long-term culture led to ESC differentiation. ID-8 reversibly maintained the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation.

References:

[1]. Miyabayashi T, Yamamoto M, Sato A, et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem, 2008, 72(5): 1242-1248.

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Chemical Properties

Cas No. 147591-46-6 SDF Download SDF
Chemical Name 1-(4-methoxyphenyl)-2-methyl-3-nitroindol-6-ol
Canonical SMILES CC1=C(C2=C(N1C3=CC=C(C=C3)OC)C=C(C=C2)O)[N+](=O)[O-]
Formula C16H14N2O4 M.Wt 298.29
Solubility >13.95mg/mL in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Background

ID-8 is a small molecule inhibitor of DYRK [1].

Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) is an enzyme that catalyzes autophosphorylation on tyrosine and serine/threonine residues.

ID-8 is a small molecule DYRK inhibitor. In HES2 cells, ID-8 (0.5 μM) increased hESC survival by 1.1%. The combination of ID-8 and Wnt3a increased survival by 1.7% and completely inhibited Wnt-induced morphological differentiation. Also, ID-8 significantly reduced the expression of differentiation marker gene GATA6, GSC, SOX17 and CDX2 induced by Wnt. In hESCs, ID-8 increased Wnt-mediated hESC proliferation and survival via inhibition of DYRKs. Also, DYRK family is direct targets of ID-8 [1]. In embryonic stem cells (ESCs), ID-8 (10 μM) stimulated proliferation in culture without MEFs, serum or LIF. The ID-8 induced ESCs showed the ability to form all three germ layer-derived tissues, such as liver, neurons and muscles. Also, ID-8 increased ESC differentiation and the levels of Nanog, Sox2 and Rex-1 [2]. In mouse ESCs cultured in serum-free medium, ID-8 maintained their self-renewal and pluripotency [3].

References:
[1].  Hasegawa K, Yasuda SY, Teo JL, et al. Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med, 2012, 1(1): 18-28.
[2].  Miyabayashi T, Yamamoto M, Sato A, et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem, 2008, 72(5): 1242-1248.
[3].  Firestone AJ, Chen JK. Controlling destiny through chemistry: small-molecule regulators of cell fate. ACS Chem Biol, 2010, 5(1): 15-34.