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HyperScribe™ All in One mRNA Synthesis Kit II Plus 2 (EZ Cap Reagent AG (3' OMe), 5-moUTP, T7, poly(A))

Catalog No.
K1068
Used for the in vitro synthesis of mRNA (EZ Cap Reagent AG (3' OMe) capped, 5-moUTP modified, poly(A) tailed)
Grouped product items
SizePriceStock Qty
25 rxns
$800.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Description

HyperScribe™ All in One mRNA Synthesis Kit II Plus 2 (EZ Cap Reagent AG (3' OMe), 5-moUTP, T7, poly(A)) is designed for quick production of EZ Cap Reagent AG (3' OMe) capped, 5-moUTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of capping reagent (EZ Cap Reagent AG (3' OMe), Catalog No. B8178) using T7 RNA Polymerase. The capping of EZ Cap Reagent AG (3' OMe) results in a Cap 1 structure and ensures high translation efficacy. The addition of 5-moUTP (Catalog No. B8061) can suppress RNA-mediated innate immune activation. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.

RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.

The kit contains sufficient reagents to carry out 25 reactions, 20 μl each time. Up to 25-180 μg of RNA can be generated with 1 μg of control template per standard reaction.

Quality Control

Quality Control & MSDS

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Components and Storage

(1) RNA Synthesis with EZ Cap Reagent AG (3' OMe), 5-moUTP

Components 25 rxns
T7 RNA Polymerase Mix 50 μl
10 × Reaction Buffer 50 μl
ATP (20 mM) 50 μl
UTP (20 mM) 37.5 μl
CTP (20 mM) 50 μl
GTP (20 mM) 25 μl
EZ Cap Reagent AG (3' OMe) (60 mM) 35 μl
5-moUTP (10 mM) 25 μl
Control Template (0.5 μg/μl) 5 μl
RNase-free H2O 0.5 ml

Store all kit components at -20°C.

(2) Tailing Reaction

Components 25 rxns
E-PAP (2 units/μl) 100 μl
5X E-PAP Buffer 600 μl
ATP Solution (100 mM) 100 μl
25 mM MnCl2 250 μl
Control DNA Template (0.5 μg/μl) 10 μl
Nuclease - free Water 2x1ml

Store all kit components at -20°C.