hyPerFUsion™ high-fidelity DNA polymerase
Quality Control & DataSheet
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Related Biological Data
Related Biological Data
Components and storage
|hyPerFUsion™ High-Fidelity DNA Polymerase||1,000 units/ml||-20°C|
|5X hyPerFUsion™ Buffer||5 X||-20°C|
|Product Source||An E. coli strain that carries the hyPerFUsion™ DNA Polymerase gene.|
hyPerFUsion™ high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, hyPerFUsion™ DNA polymerases produce PCR products with very high speed and accuracy. Also, hyPerFUsion™ DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. hyPerFUsion™ high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. hyPerFUsion™ DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), hyPerFUsion™ is one of the most accurate thermostable polymerases available. hyPerFUsion™ DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
hyPerFUsion™ DNA Polymerase is supplied with standard 5X hyPerFUsion™ Buffer, which can be used for complex or GC-rich templates. Reactions can also be optimized using the provided DMSO.
1. High fidelity—up to 50x more accurate than Taq; up to 6x more accurate than Pfu DNA polymerase.
2. Enhanced robustness—fewer reaction failures and minimal optimization.
3. Improved yields—high product yields with minimal enzyme amounts (0.5–1 U/50 μL reaction).
4. High speed—increased processivity allows shorter reaction times (10X faster than Pfu).
5. Versatile – Can be used for routine PCR as well as long or difficult (GC-rich) templates.