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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tyramide signal amplification (TSA) technology can be used to detect low-abundance targets in tissue and cell immunofluorescence (IF), immunochemistry, in situ hybridization (ISH), and more, increasing signal sensitivity by about 100-fold. The fluorescently labeled tyramide molecule is catalyzed by the secondary antibody-labeled HRP in the presence of H2O2, resulting in a large number of enzymatic reactions, which make the fluorescent molecules bind to the protein residues around the tissue at the antigen-antibody binding site, forming a large number of fluorescent molecule deposits to achieve signal amplification. The labeling process is rapid (less than 10 minutes) and the deposition marks can be viewed directly under a standard or confocal microscope. TSA fluorescence kits can also be used in conjunction with traditional immunofluorescence methods for multicolor imaging, or two or more tyrosamide reactions can be performed sequentially to label different targets on a sample. The use of TSA reagents can significantly improve signal sensitivity while maintaining stable specificity and resolution compared to normal experiments, and in addition, TSA reagents can significantly reduce the consumption of primary antibodies or probes.
The fluorescently labeled signal of this kit is HyperFluorTM 594 (591 nm/617 nm) and can be easily detected with a standard standard red/Texas Red filter.
Components
200 slides
400 slides
Storage
1X Amplification Diluent
20 mL
40 mL
4°C
HyperFluorTM 594 Tyramide (200×，dry, dissolve in 100 μL DMSO)
1 tube
2 tubes
-20°C away from light
Blocking Reagent (BSA)
6 g
12 g
Shipping Blue ice
Shelf life: 12 months
