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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Human B cell culture and expansion kit is for activation and expansion of human B cells. Human monoclonal antibodies isolated for specific antibodies are widely used in immunity studies, historical studies and therapeutic development. Memory B cells derived from peripheral blood will produce high concentration of IgG in the supernatant under the stimulation of CD40L with other growth factors [1]. The B Cell Expansion Kit contains special treated feeder cells, expressing CD40L in activated form as well as human cell factors A and B potently inducing antibody-secret. The monoclonal antibody screening can be completed within 2 weeks with the help of the kit.
Reference:
[1] J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013).