In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
GGsTop is a novel is a novel, highly selective, and irreversible γ-glutamyl transpeptidase (GGT) inhibitor with Ki of 0.17 mM. 
GGT plays an important role in the metabolism of plasma glutathione (GSH) and its S-conjugates via the cleavage of the γ-glutamyl amide bond by hydrolysis and/or transpeptidation. GGT exists in the lung as a soluble enzyme in association with surfactant phospholipids and controls turnover of the extracellular pool of glutathione in LLF (lung lining ﬂuid). In the human enzyme, a specific residue in the Cys - Gly binding site played a critical role in recognizing the Cys – Gly moiety or the acceptor molecules by interacting with the C-terminal carboxy group, whereas the Cys side chain and the Cys - Gly amide bond were not recognized significantly. GGsTop was found for its potency against GGT in a series of analogues designed for structure-activity relationships. [1, 2]
The inactivation rates of E. coli and human GGT by GGsTop were studied. GGsTop showed a good potency against GGT in both species. Furthermore, the kinetics of GGsTop for human GGT was also measured and its Ki value is 0.17 mM. 
In the IL-13 model, mice treated with IL-13 and GGsTop exhibit a lung inﬂammatory response similar to that of mice administrated with IL-13 alone. However, mice treated with IL-13 and GGsTop exhibit attenuation of methacholine-stimulated airway hyper-reactivity, inhibitory effect of Muc5ac and Muc5b gene induction, decreased epithelial cell mucous accumulation in the airway and a 4-fold increase in LLF glutathione content compared to mice in IL-13 group. The associated increase in LLF glutathione can protect lung airway epithelial cells against oxidant injury associated with inﬂammation in asthma. Moreover, ischemic acute kidney injury (AKI) was induced by occlusion of the left renal artery and vein for 45 min followed by reperfusion 2 weeks after contralateral nephrectomy. Renal function in control significantly decreased at 1 day after reperfusion. Rats administrated with GGsTop at doses of 1 and 10 mg per kg i.v. 5 min before ischemia attenuated the I/R-induced renal dysfunction in a dose-dependent manner. Histopathological evaluation of the kidney of AKI rats revealed severe renal damages, whereas they were significantly suppressed by the GGsTop treatment. [2, 3]
. Han, Liyou, et al. "Design, synthesis, and evaluation of γ-phosphono diester analogues of glutamate as highly potent inhibitors and active site probes of γ-glutamyl transpeptidase." Biochemistry 46.5 (2007): 1432-1447.
. Yamamoto, Shinya, et al. "Preventive effect of GGsTop, a novel and selective γ-glutamyl transpeptidase inhibitor, on ischemia/reperfusion-induced renal injury in rats." Journal of Pharmacology and Experimental Therapeutics 339.3 (2011): 945-951.
. Tuzova, Marina, et al. "Inhibiting lung lining fluid glutathione metabolism with GGsTop as a novel treatment for asthma." Frontiers in pharmacology 5 (2014)
- 1. Jia-Ru Wei, Jun Dong, et al. "Cancer-associated fibroblasts-derived gamma-glutamyltransferase 5 promotes tumor growth and drug resistance in lung adenocarcinoma." Aging (Albany NY). 2020 Jul 8;12(13):13220-13233. PMID:32640421
- 2. LeiJianga, DanGuoa, et al. "Sensitive and selective SERS probe for detecting the activity of γ-glutamyl transpeptidase in serum." Analytica Chimica Acta. Available online 21 November 2019.
|Physical Appearance||White solid|
|Storage||Store at -20°C|
|Solubility||<33.13mg/ml in H2O; <33.13mg/ml in DMSO|
|Chemical Name||(S)-2-amino-4-((S)-(3-(carboxymethyl)phenoxy)(methoxy)phosphoryl)butanoic acid|
|Canonical SMILES||O=[[email protected]](OC)(CC[[email protected]@H](C(O)=O)N)OC1=CC=CC(CC(O)=O)=C1|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|