Toggle Nav

Geneticin, G-418 Sulfate

Catalog No.
A2513
Aminoglycosidic antibiotic
Grouped product items
SizePriceStock Qty
1g
$70.00
In stock
5g
$210.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

G-418 is an elongation inhibitor of 80 S ribosomes [1].

G418 is an aminoglycoside antibiotic, it has a wide spectrum of activity and is active against bacteria, yeasts, fungi, algae, plant and even animal cells. G418 is usually used as a cloning selection in pro- and eukaryotes at concentrations from 1 to 300 g/ml. In the studies of antibiotic resistance in clinical isolates of bactenia, it is found that all strains carrying 3'-O-aminoglycoside phosphotransferases are resistant to G418. Among these phosphotransferases, type I and type II are encoded by the transposable elements TnS and Tn601, respectively [2].

Apart from the selector function, G418 is also found to have antiviral activity against bovine viral diarrhea virus (BVDV). G418 prevents the cytopathic effect (CPE) resulting from DENV-2 infection of BHK cells, in a dose-dependent manner with an EC50 value of 3±0.4 g/ml [3].

References:
[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.
[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092.
[3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt692.71
Cas No.108321-42-2
FormulaC20H40N4O10·2H2SO4
SynonymsAntibiotic G418
Solubilityinsoluble in DMSO; ≥240.4 mg/ml in H2O
Chemical Name2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
SDFDownload SDF
Canonical SMILESCC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1, 2]:

Cell lines

DENV-2-infected BHK cells

Preparation method

This compound is limited soluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

1–300 μg/ml, 120 h

Applications

Geneticin (1–300 μg/ml) inhibited many pro- and eukaryotes. Geneticin reduced viability of BHK cells with a CC50 value of 165 ± 5 μg/ml. Geneticin prevented DENV-induced CPE and increased cell viability of infected cells with an EC50 value of ~3 ± 0.4 μg/ml. Geneticin inhibited the yield of viral titers of DENV-2 even after 72 hpi with an EC50 value of 2 ± 0.1 μg/ml and an EC90 value of about 20 ± 2 μg/ml. Geneticin inhibited the number of plaques produced by the virus, and significantly reduced the sizes of the remaining plaques.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.

[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092. [3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.

Biological Activity

Description G-418 is an aminoglycoside antibiotic.
Targets 80 S ribosomes          
IC50            

Quality Control