EZ Cap™ Human PTEN mRNA (ψUTP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PTEN (deletion of phosphatase and tensin homologues on chromosome 10) is a tumor suppressor gene that resists the action of PI3K, which is a key enzyme for anti-apoptotic, pro-tumorigenic Akt activation.
EZ Cap™ Human PTEN mRNA (ψUTP) is provided at a concentration of 1mg/ml. It is co-transcriptional capped which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). ψUTP and poly (A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
| mRNA Length | 1467 nucleotides |
| Concentration | 1 mg/mL |
| Buffer | 1 mM Sodium Citrate, pH 6.4 |
| Storage | -40°C or below |
| General tips | Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
| Shipping Condition | ship with dry ice |