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DL-threo-2-methylisocitrate sodium
Substrate of isocitrate lyase 1(ICL1)

Catalog No.B1310
Size Price Stock
1000mg Please Inquire Please Inquire

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Sample solution is provided at 25 µL, 10mM.

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Chemical structure

DL-threo-2-methylisocitrate sodium

DL-threo-2-methylisocitrate sodium Dilution Calculator

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Chemical Properties

Cas No. N/A SDF Download SDF
Synonyms N/A
Chemical Name sodium (2R,3S)-3-hydroxybutane-1,2,3-tricarboxylate
Canonical SMILES C[[email protected]](C([O-])=O)(O)[[email protected]@](C([O-])=O)([H])CC([O-])=O.C[[email protected]](C([O-])=O)(O)[[email protected]@](C([O-])=O)([H])CC([O-])=O.[Na+].[Na+].[Na+].[Na+].[Na+].[Na+]
Formula C14H14Na6O14 M.Wt 544.19
Solubility Soluble in DMSO Storage Store at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

DL-threo-2-methylisocitrate sodium is a substrate of isocitratelyase 1(ICL1).

ICL1 had dual activity for both threo-D(s)L(s)-isocitrate (ICA) and DL-threo-2-methylisocitrate (MICA). Using Michaelis-Menten non-linear least squares fit, the Km of purified recombinant ICL1 for threo-D(s)L(s)-isocitrate (ICA) was determined to be 188 mM with akcatof 5.24 s-1 .The Km of ICL2 for MICA was greater than 15 mM which was too high to be determained accurately due to the limitations of the solubility of MICA. The Kcat/Km for ICL1, a measureforcatalyticefficiency, was 2.79 × 104 M-1 s-1 and 1.74 ×103 M-1 s-1, for ICA and MICA respectively.

Reference:
[1].  Gould TA1,van de Langemheen H,Muoz-Elías EJ,McKinney JD,Sacchettini JC. Dual role of isocitratelyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis.Mol Microbiol.2006 Aug;61(4):940-7.